Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
Date of Issue2017
School of Physical and Mathematical Sciences
It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes.
Bioorganic & Medicinal Chemistry Letters
© 2016 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioorganic & Medicinal Chemistry Letters, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bmcl.2016.11.088].