dc.contributor.authorYam, Gary Hin-Fai
dc.contributor.authorWilliams, Geraint P.
dc.contributor.authorSetiawan, Melina
dc.contributor.authorYusoff, Nur Zahirah Binte M.
dc.contributor.authorLee, Xiao-wen
dc.contributor.authorHtoon, Hla Myint
dc.contributor.authorZhou, Lei
dc.contributor.authorFuest, Matthias
dc.contributor.authorMehta, Jodhbir Singh
dc.identifier.citationYam, G. H.-F., Williams, G. P., Setiawan, M., Yusoff, N. Z. B. M., Lee, X.-w., Htoon, H. M., et al. (2017). Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts. Scientific Reports, 7, 45396-.en_US
dc.description.abstractLaser refractive surgeries reshape corneal stroma to correct refractive errors, but unavoidably affect corneal nerves. Slow nerve regeneration and atypical neurite morphology cause desensitization and neuro-epitheliopathy. Following injury, surviving corneal stromal keratocytes (CSKs) are activated to stromal fibroblasts (SFs). How these two different cell types influence nerve regeneration is elusive. Our study evaluated the neuro-regulatory effects of human SFs versus CSKs derived from the same corneal stroma using an in vitro chick dorsal root ganglion model. The neurite growth was assessed by a validated concentric circle intersection count method. Serum-free conditioned media (CM) from SFs promoted neurite growth dose-dependently, compared to that from CSKs. We detected neurotrophic and pro-inflammatory factors (interleukin-8, interleukin-15, monocyte chemoattractant protein-1, eotaxin, RANTES) in SFCM by Bio-Plex Human Cytokine assay. More than 130 proteins in SFCM and 49 in CSKCM were identified by nanoLC-MS/MS. Proteins uniquely present in SFCM had reported neuro-regulatory activities and were predicted to regulate neurogenesis, focal adhesion and wound healing. Conclusively, this was the first study showing a physiological relationship between nerve growth and the metabolically active SFs versus quiescent CSKs from the same cornea source. The dose-dependent effect on neurite growth indicated that nerve regeneration could be influenced by SF density.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.format.extent15 p.en_US
dc.relation.ispartofseriesScientific Reportsen_US
dc.rights© 2017 The Author(s) (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.subjectCell growthen_US
dc.subjectTranslational researchen_US
dc.titleNerve regeneration by human corneal stromal keratocytes and stromal fibroblastsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.versionPublished versionen_US

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