dc.contributor.authorRousseau, Matthieu
dc.contributor.authorGoh, Hwee Mian Sharon
dc.contributor.authorHolec, Sarah
dc.contributor.authorAlbert, Matthew L.
dc.contributor.authorWilliams, Rohan B.H.
dc.contributor.authorIngersoll, Molly A.
dc.contributor.authorKline, Kimberly A.
dc.identifier.citationRousseau, M., Goh, H. M. S., Holec, S., Albert, M. L., Williams, R. B. H., Ingersoll, M. A., et al. (2016). Bladder catheterization increases susceptibility to infection that can be prevented by prophylactic antibiotic treatment. JCI Insight, 1(15), e88178-.en_US
dc.description.abstractCatheter-associated urinary tract infections (CAUTI) are the most common hospital-associated infections. Here, we report that bladder catheterization initiated a persistent sterile inflammatory reaction within minutes of catheter implantation. Catheterization resulted in increased expression of genes associated with defense responses and cellular migration, with ensuing rapid and sustained innate immune cell infiltration into the bladder. Catheterization also resulted in hypersensitivity to Enterococcus faecalis and uropathogenic Escherichia coli (UPEC) infection, in which colonization was achieved using an inoculum 100-fold lower than the ID90 for infection of an undamaged urothelium with the same uropathogens. As the time of catheterization increased, however, colonization by the Gram-positive uropathogen E. faecalis was reduced, whereas catheterization created a sustained window of vulnerability to infection for Gram-negative UPEC over time. As CAUTI contributes to poorer patient outcomes and increased health care expenditures, we tested whether a single prophylactic antibiotic treatment, concurrent with catheterization, would prevent infection. We observed that antibiotic treatment protected against UPEC and E. faecalis bladder and catheter colonization as late as 6 hours after implantation. Thus, our study has revealed a simple, safe, and immediately employable intervention, with the potential to decrease one of the most costly hospital-incurred infections, thereby improving patient and health care economic outcome.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent13 p.en_US
dc.relation.ispartofseriesJCI Insighten_US
dc.rights© 2016 American Society for Clinical Investigation (ASCI). This paper was published in JCI Insight and is made available as an electronic reprint (preprint) with permission of American Society for Clinical Investigation (ASCI). The published version is available at: [http://dx.doi.org/10.1172/jci.insight.88178]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en_US
dc.titleBladder catheterization increases susceptibility to infection that can be prevented by prophylactic antibiotic treatmenten_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen_US

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