dc.contributor.authorHansen, Finja C.
dc.contributor.authorStrömdahl, Ann-Charlotte
dc.contributor.authorMörgelin, Matthias
dc.contributor.authorSchmidtchen, Artur
dc.contributor.authorvan der Plas, Mariena J. A.
dc.date.accessioned2017-07-24T03:04:52Z
dc.date.available2017-07-24T03:04:52Z
dc.date.issued2017
dc.identifier.citationHansen, F. C., Strömdahl, A.-C., Mörgelin, M., Schmidtchen, A., & van der Plas, M. J. A. (2017). Thrombin-Derived Host-Defense Peptides Modulate Monocyte/Macrophage Inflammatory Responses to Gram-Negative Bacteria. Frontiers in Immunology, 8, 843-.en_US
dc.identifier.urihttp://hdl.handle.net/10220/43423
dc.description.abstractHost-defense peptides play a fundamental role in the innate immune system by modulating inflammatory responses. Previously, it was shown that the thrombin derived host-defense peptide GKY25 inhibits LPS-induced responses of monocytes and macrophages in vitro, ex vivo, and in vivo. In this study, the effect of GKY25 on the interaction of monocytes/macrophages with Gram-negative bacteria was explored. Electron microscopy analysis showed that fibrin slough from non-healing wounds, colonized with Staphylococcus aureus and Pseudomonas aeruginosa, contains C-terminal thrombin epitopes associated with these bacteria extracellularly and in phagosomes of leukocytes. Live imaging of RAW 264.7 cell cultures showed binding of GKY25 to Escherichia coli BioParticles extracellularly, and colocalization intracellularly. Although peptide binding did not alter the rate of phagocytosis, GKY25 reduced NF-κB/AP-1 activation and subsequent cytokine release in response to both heat-killed and live bacteria. Notably, preincubation of RAW 264.7 cells with peptide did increase BioParticle uptake in a dose-dependent manner. Taken together, the thrombin-derived host-defense peptide GKY25 binds to bacteria extracellularly and colocalizes with bacteria intracellularly, thereby reducing pro-inflammatory responses.en_US
dc.format.extent11 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesFrontiers in Immunologyen_US
dc.rights© 2017 Hansen, Strömdahl, Mörgelin, Schmidtchen and van der Plas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectHost-defense peptidesen_US
dc.subjectGram-negative bacteriaen_US
dc.titleThrombin-Derived Host-Defense Peptides Modulate Monocyte/Macrophage Inflammatory Responses to Gram-Negative Bacteriaen_US
dc.typeJournal Article
dc.contributor.schoolLee Kong Chian School of Medicineen_US
dc.identifier.doihttp://dx.doi.org/10.3389/fimmu.2017.00843
dc.description.versionPublished versionen_US


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