dc.contributor.authorLudwig, Alexander
dc.contributor.authorNguyen, Tra Huong
dc.contributor.authorLeong, Daniel
dc.contributor.authorRavi, Laxmi Iyer
dc.contributor.authorHuan, Tan Boon
dc.contributor.authorSandin, Sara
dc.contributor.authorSugrue, Richard J.
dc.date.accessioned2017-07-25T08:12:22Z
dc.date.available2017-07-25T08:12:22Z
dc.date.issued2017
dc.identifier.citationLudwig, A., Nguyen, T. H., Leong, D., Ravi, L. I., Huan, T. B., Sandin, S., et al. (2017). Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly. Journal of Cell Science, 130, 1037-1050.en_US
dc.identifier.issn0021-9533en_US
dc.identifier.urihttp://hdl.handle.net/10220/43442
dc.description.abstractRespiratory syncytial virus (RSV) is an enveloped virus that assembles into filamentous virus particles on the surface of infected cells. Morphogenesis of RSV is dependent upon cholesterol-rich (lipid raft) membrane microdomains, but the specific role of individual raft molecules in RSV assembly is not well defined. Here, we show that RSV morphogenesis occurs within caveolar membranes and that both caveolin-1 and cavin-1 (also known as PTRF), the two major structural and functional components of caveolae, are actively recruited to and incorporated into the RSV envelope. The recruitment of caveolae occurred just prior to the initiation of RSV filament assembly, and was dependent upon an intact actin network as well as a direct physical interaction between caveolin-1 and the viral G protein. Moreover, cavin-1 protein levels were significantly increased in RSV-infected cells, leading to a virus-induced change in the stoichiometry and biophysical properties of the caveolar coat complex. Our data indicate that RSV exploits caveolae for its assembly, and we propose that the incorporation of caveolae into the virus contributes to defining the biological properties of the RSV envelope.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent14 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesJournal of Cell Scienceen_US
dc.rights© 2017 The Author(s) (published by The Company of Biologists Ltd). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en_US
dc.subjectCaveolaeen_US
dc.subjectCaveolinen_US
dc.titleCaveolae provide a specialized membrane environment for respiratory syncytial virus assemblyen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1242/jcs.198853
dc.description.versionPublished versionen_US


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