Dynamic in vivo imaging of small animal brain using pulsed laser diode-based photoacoustic tomography system
Upputuri, Paul Kumar
Date of Issue2017
School of Chemical and Biomedical Engineering
We demonstrate dynamic in vivo imaging using a low-cost portable pulsed laser diode based photoacoustic tomography system. The system takes advantage of an 803 nm pulsed laser diode having high repetition rate ~7000 Hz combined with a fast-scanning single-element ultrasound transducer leading to a 5 seconds cross-sectional imaging. Cortical vasculature is imaged in scan-time of 5 seconds with high signal-to-noise ratio ~48. To examine the ability for dynamic imaging, we monitored the fast uptake and clearance process of indo-cyanine green in the rat brain. The system will find applications to study neuro-functional activities, characterization of pharmacokinetic and bio-distribution profiles in the development process of drugs or imaging agents.
In vivo imaging
In vivo imaging
Journal of Biomedical Optics
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE). This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-Optical Instrumentation Engineers (SPIE). The published version is available at: [http://dx.doi.org/10.1117/1.JBO.22.9.090501]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.