dc.contributor.authorVenkatesh, Mayandi
dc.contributor.authorBarathi, Veluchamy Amutha
dc.contributor.authorGoh, Eunice Tze Leng
dc.contributor.authorAnggara, Raditya
dc.contributor.authorFazil, Mobashar Hussain Urf Turabe
dc.contributor.authorNg, Alice Jie Ying
dc.contributor.authorHarini, Sriram
dc.contributor.authorAung, Thet Tun
dc.contributor.authorFox, Stephen John
dc.contributor.authorLiu, Shouping
dc.contributor.authorYang, Liang
dc.contributor.authorBarkham, Timothy Mark Sebastian
dc.contributor.authorLoh, Xian Jun
dc.contributor.authorVerma, Navin Kumar
dc.contributor.authorBeuerman, Roger W.
dc.contributor.authorLakshminarayanan, Rajamani
dc.date.accessioned2017-10-10T08:38:29Z
dc.date.available2017-10-10T08:38:29Z
dc.date.issued2017
dc.identifier.citationVenkatesh, M., Barathi, V. A., Goh, E. T. L., Anggara, R., Fazil, M. H. U. T., Ng, A. J. Y., et al. (2017). Antimicrobial Activity and Cell Selectivity of Synthetic and Biosynthetic Cationic Polymers. Antimicrobial Agents and Chemotherapy, 61(10), e00469-17-.en_US
dc.identifier.issn0066-4804en_US
dc.identifier.urihttp://hdl.handle.net/10220/43854
dc.description.abstractThe mammalian and microbial cell selectivity of synthetic and biosynthetic cationic polymers has been investigated. Among the polymers with peptide backbones, polymers containing amino side chains display greater antimicrobial activity than those with guanidine side chains, whereas ethylenimines display superior activity over allylamines. The biosynthetic polymer ε-polylysine (εPL) is noncytotoxic to primary human dermal fibroblasts at concentrations of up to 2,000 μg/ml, suggesting that the presence of an isopeptide backbone has greater cell selectivity than the presence of α-peptide backbones. Both εPL and linear polyethylenimine (LPEI) exhibit bactericidal properties by depolarizing the cytoplasmic membrane and disrupt preformed biofilms. εPL displays broad-spectrum antimicrobial properties against antibiotic-resistant Gram-negative and Gram-positive strains and fungi. εPL elicits rapid bactericidal activity against both Gram-negative and Gram-positive bacteria, and its biocompatibility index is superior to those of cationic antiseptic agents and LPEI. εPL does not interfere with the wound closure of injured rabbit corneas. In a rabbit model of bacterial keratitis, the topical application of εPL (0.3%, wt/vol) decreases the bacterial burden and severity of infections caused by Pseudomonas aeruginosa and Staphylococcus aureus strains. In vivo imaging studies confirm that εPL-treated corneas appeared transparent and nonedematous compared to untreated infected corneas. Taken together, our results highlight the potential of εPL in resolving topical microbial infections.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.description.sponsorshipMOH (Min. of Health, S’pore)en_US
dc.format.extent15 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesAntimicrobial Agents and Chemotherapyen_US
dc.rights© 2017 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.subjectAntimicrobial Activityen_US
dc.subjectCationic Polymersen_US
dc.titleAntimicrobial Activity and Cell Selectivity of Synthetic and Biosynthetic Cationic Polymersen_US
dc.typeJournal Article
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolLee Kong Chian School of Medicineen_US
dc.identifier.doihttp://dx.doi.org/10.1128/AAC.00469-17
dc.description.versionPublished versionen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record