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https://hdl.handle.net/10356/85809
Title: | Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation | Authors: | Tay, Hui Min Kharel, Sharad Dalan, Rinkoo Chen, Zhijie Joshua Tan, Kah Kee Boehm, Bernhard Otto Loo, Say Chye Joachim Hou, Han Wei |
Keywords: | Sub-micrometer Particles Drug Release |
Issue Date: | 2017 | Source: | Tay, H. M., Kharel, S., Dalan, R., Chen, Z. J., Tan, K. K., Boehm, B. O., et al. (2017). Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation. NPG Asia Materials, 9(9), e434-. | Series/Report no.: | NPG Asia Materials | Abstract: | Efficient separation of sub-micrometer synthetic or biological components is imperative in particle-based drug delivery systems and purification of extracellular vesicles for point-of-care diagnostics. Herein, we report a novel phenomenon in spiral inertial microfluidics, in which the particle transient innermost distance (Dinner) varies with size during Dean vortices-induced migration and can be utilized for small microparticle (MP) separation; aptly termed as high-resolution Dean flow fractionation (HiDFF). The developed technology was optimized using binary bead mixtures (1–3 μm) to achieve ~100- to 1000-fold enrichment of smaller particles. We demonstrated tunable size fractionation of polydispersed drug-loaded poly(lactic-co-glycolic acid) particles for enhanced drug release and anti-tumor effects. As a proof-of-concept for microvesicles studies, circulating extracellular vesicles/MPs were isolated directly from whole blood using HiDFF. Purified MPs exhibited well-preserved surface morphology with efficient isolation within minutes as compared with multi-step centrifugation. In a cohort of type 2 diabetes mellitus subjects, we observed strong associations of immune cell-derived MPs with cardiovascular risk factors including body mass index, carotid intima-media thickness and triglyceride levels (P<0.05). Overall, HiDFF represents a key technological progress toward high-throughput, single-step purification of engineered or cell-derived MPs with the potential for quantitative MP-based health profiling. | URI: | https://hdl.handle.net/10356/85809 http://hdl.handle.net/10220/43855 |
ISSN: | 1884-4057 | DOI: | 10.1038/am.2017.175 | Schools: | Lee Kong Chian School of Medicine (LKCMedicine) School of Materials Science & Engineering |
Research Centres: | Singapore Centre for Environmental Life Sciences and Engineering | Rights: | © 2017 The Author(s) (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | LKCMedicine Journal Articles |
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Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation.pdf | 3.04 MB | Adobe PDF | View/Open |
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