Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86521
Title: Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells
Authors: Trott, Jamie
Tan, Ee Kim
Ong, Sheena
Titmarsh, Drew M.
Denil, Simon L.I.J.
Giam, Maybelline
Wong, Cheng Kit
Wang, Jiaxu
Shboul, Mohammad
Eio, Michelle
Cooper-White, Justin
Cool, Simon M.
Rancati, Giulia
Stanton, Lawrence W.
Reversade, Bruno
Dunn, Norris Ray
Keywords: Culture Conditions
Pancreatic Progenitors
Issue Date: 2017
Source: Trott, J., Tan, E. K., Ong, S., Titmarsh, D. M., Denil, S. L., Giam, M., et al. (2017). Long-Term Culture of Self-renewing Pancreatic Progenitors Derived from Human Pluripotent Stem Cells. Stem Cell Reports, 8(6), 1675-1688.
Series/Report no.: Stem Cell Reports
Abstract: Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse genetic backgrounds. We have developed culture conditions that support long-term self-renewal of human multipotent pancreatic progenitors, which are developmentally more proximal to the specialized cells of the adult pancreas. These cultured pancreatic progenitor (cPP) cells express key pancreatic transcription factors, including PDX1 and SOX9, and exhibit transcriptomes closely related to their in vivo counterparts. Upon exposure to differentiation cues, cPP cells give rise to pancreatic endocrine, acinar, and ductal lineages, indicating multilineage potency. Furthermore, cPP cells generate insulin+ β-like cells in vitro and in vivo, suggesting that they offer a convenient alternative to pluripotent cells as a source of adult cell types for modeling pancreatic development and diabetes.
URI: https://hdl.handle.net/10356/86521
http://hdl.handle.net/10220/44039
ISSN: 2213-6711
DOI: http://dx.doi.org/10.1016/j.stemcr.2017.05.019
Rights: © 2017 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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