dc.contributor.authorEdupuganti, Raghu Ram
dc.contributor.authorHarikumar, Arigela
dc.contributor.authorAaronson, Yair
dc.contributor.authorBiran, Alva
dc.contributor.authorSailaja, Badi Sri
dc.contributor.authorNissim-Rafinia, Malka
dc.contributor.authorAzad, Gajendra Kumar
dc.contributor.authorCohen, Malkiel A.
dc.contributor.authorPark, Jung Eun
dc.contributor.authorShivalila, Chikdu S.
dc.contributor.authorMarkoulaki, Styliani
dc.contributor.authorSze, Siu Kwan
dc.contributor.authorJaenisch, Rudolf
dc.contributor.authorMeshorer, Eran
dc.date.accessioned2017-11-15T03:34:29Z
dc.date.available2017-11-15T03:34:29Z
dc.date.issued2017
dc.identifier.citationEdupuganti, R. R., Harikumar, A., Aaronson, Y., Biran, A., Sailaja, B. S., Nissim-Rafinia, M., et al. (2017). Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation. Stem Cell Reports, 9(4), 1291-1303.en_US
dc.identifier.issn2213-6711en_US
dc.identifier.urihttp://hdl.handle.net/10220/44040
dc.description.abstractEmbryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage.en_US
dc.format.extent13 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesStem Cell Reportsen_US
dc.rights© 2017 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.subjectSETen_US
dc.subjectEmbryonic Stem Cellsen_US
dc.titleAlternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiationen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.stemcr.2017.08.021
dc.description.versionPublished versionen_US


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