dc.contributor.authorShi, Pujiang
dc.contributor.authorLaude, Augustinus
dc.contributor.authorYeong, Wai Yee
dc.identifier.citationShi, P., Laude, A., & Yeong, W. Y. (2017). Investigation of cell viability and morphology in 3D bio-printed alginate constructs with tunable stiffness. Journal of Biomedical Materials Research Part A, 105(4), 1009-1018.en_US
dc.description.abstractIn this article, mouse fibroblast cells (L929) were seeded on 2%, 5%, and 10% alginate hydrogels, and they were also bio-printed with 2%, 5%, and 10% alginate solutions individually to form constructs. The elastic and viscous moduli of alginate solutions, their interior structure and stiffness, interactions of cells and alginate, cell viability, migration and morphology were investigated by rheometer, MTT assay, scanning electron microscope (SEM), and fluorescent microscopy. The three types of bio-printed scaffolds of distinctive stiffness were prepared, and the seeded cells showed robust viability either on the alginate hydrogel surfaces or in the 3D bio-printed constructs. Majority of the proliferated cells in the 3D bio-printed constructs weakly attached to the surrounding alginate matrix. The concentration of alginate solution and hydrogel stiffness influenced cell migration and morphology, moreover the cells formed spheroids in the bio-printed 10% alginate hydrogel construct.en_US
dc.format.extent22 p.en_US
dc.relation.ispartofseriesJournal of Biomedical Materials Research Part Aen_US
dc.rights© 2017 Wiley Periodicals, Inc. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biomedical Materials Research Part A, Wiley Periodicals, Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/jbm.a.35971].en_US
dc.titleInvestigation of cell viability and morphology in 3D bio-printed alginate constructs with tunable stiffnessen_US
dc.typeJournal Article
dc.contributor.researchSingapore Centre for 3D Printingen_US
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.description.versionAccepted versionen_US

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