Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86550
Title: Investigation of cell viability and morphology in 3D bio-printed alginate constructs with tunable stiffness
Authors: Shi, Pujiang
Laude, Augustinus
Yeong, Wai Yee
Keywords: Alginate
Bio-printing
Issue Date: 2017
Source: Shi, P., Laude, A., & Yeong, W. Y. (2017). Investigation of cell viability and morphology in 3D bio-printed alginate constructs with tunable stiffness. Journal of Biomedical Materials Research Part A, 105(4), 1009-1018.
Series/Report no.: Journal of Biomedical Materials Research Part A
Abstract: In this article, mouse fibroblast cells (L929) were seeded on 2%, 5%, and 10% alginate hydrogels, and they were also bio-printed with 2%, 5%, and 10% alginate solutions individually to form constructs. The elastic and viscous moduli of alginate solutions, their interior structure and stiffness, interactions of cells and alginate, cell viability, migration and morphology were investigated by rheometer, MTT assay, scanning electron microscope (SEM), and fluorescent microscopy. The three types of bio-printed scaffolds of distinctive stiffness were prepared, and the seeded cells showed robust viability either on the alginate hydrogel surfaces or in the 3D bio-printed constructs. Majority of the proliferated cells in the 3D bio-printed constructs weakly attached to the surrounding alginate matrix. The concentration of alginate solution and hydrogel stiffness influenced cell migration and morphology, moreover the cells formed spheroids in the bio-printed 10% alginate hydrogel construct.
URI: https://hdl.handle.net/10356/86550
http://hdl.handle.net/10220/44103
ISSN: 1549-3296
DOI: 10.1002/jbm.a.35971
Rights: © 2017 Wiley Periodicals, Inc. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biomedical Materials Research Part A, Wiley Periodicals, Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/jbm.a.35971].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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