Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins
Mohamed, Gadisti Aisha
Date of Issue2017
Lee Kong Chian School of Medicine (LKCMedicine)
Optical silencing of activity provides a way to test the necessity of neurons in behaviour. Two light-gated anion channels, GtACR1 and GtACR2, have recently been shown to potently inhibit activity in cultured mammalian neurons and in Drosophila. Here, we test the usefulness of these channels in larval zebrafish, using spontaneous coiling behaviour as the assay.
© 2017 The Author(s) (BioMed Central). This paper was published in BMC Biology and is made available as an electronic reprint (preprint) with permission of The Author(s) (BioMed Central). The published version is available at: [http://dx.doi.org/10.1186/s12915-017-0430-2]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.