dc.contributor.authorLuo, Yuemei
dc.contributor.authorCui, Dongyao
dc.contributor.authorYu, Xiaojun
dc.contributor.authorBo, En
dc.contributor.authorWang, Xianghong
dc.contributor.authorWang, Nanshuo
dc.contributor.authorBraganza, Cilwyn S.
dc.contributor.authorChen, Shufen
dc.contributor.authorLiu, Xinyu
dc.contributor.authorXiong, Qiaozhou
dc.contributor.authorChen, Si
dc.contributor.authorChen, Shi
dc.contributor.authorLiu, Linbo
dc.identifier.citationLuo, Y., Cui, D., Yu, X., Bo, E., Wang, X., Wang, N., et al. (2018). Endomicroscopic optical coherence tomography for cellular resolution imaging of gastrointestinal tracts. Journal of Biophotonics, in press.en_US
dc.description.abstractOur ability to detect neoplastic changes in gastrointestinal (GI) tracts is limited by the lack of an endomicroscopic imaging tool that provides cellular-level structural details of GI mucosa over a large tissue area. In this article, we report a fiber-optic-based micro-optical coherence tomography (μOCT) system and demonstrate its capability to acquire cellular-level details of GI tissue through circumferential scanning. The system achieves an axial resolution of 2.48 μm in air and a transverse resolution of 4.8 μm with a depth-of-focus (DOF) of ~150 μm. To mitigate the issue of limited DOF, we used a rigid sheath to maintain a circular lumen and center the distal-end optics. The sensitivity is tested to be 98.8 dB with an illumination power of 15.6 mW on the sample. With fresh swine colon tissues imaged ex vivo, detailed structures such as crypt lumens and goblet cells can be clearly resolved, demonstrating that this fiber-optic μOCT system is capable of visualizing cellular-level morphological features. We also demonstrate that time-lapsed frame averaging and imaging speckle reduction are essential for clearly visualizing cellular-level details. Further development of a clinically viable μOCT endomicroscope is likely to improve the diagnostic outcome of GI cancers.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.format.extent17 p.en_US
dc.relation.ispartofseriesJournal of Biophotonicsen_US
dc.rights© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biophotonics, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/jbio.201700141].en_US
dc.subjectOptical Coherence Tomographyen_US
dc.subjectEndoscopic Imagingen_US
dc.titleEndomicroscopic optical coherence tomography for cellular resolution imaging of gastrointestinal tractsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolSchool of Electrical and Electronic Engineeringen_US
dc.description.versionAccepted versionen_US

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