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Title: Protecting microRNAs from RNase degradation with steric DNA nanostructures
Authors: Qian, H.
Tay, Chor Yong
Setyawati, M. I.
Chia, S. L.
Lee, D. S.
Leong, D. T.
Keywords: DNA
Issue Date: 2017
Source: Qian, H., Tay, C. Y., Setyawati, M. I., Chia, S. L., Lee, D. S., & Leong, D. T. (2017). Protecting microRNAs from RNase degradation with steric DNA nanostructures. Chemical Science, 8(2), 1062-1067.
Series/Report no.: Chemical Science
Abstract: Tumor suppressive microRNAs are potent molecules that might cure cancer, one day. Despite the many advanced strategies for delivery of these microRNAs to the cell, there are few therapeutic microRNAs in clinical use. Progress in microRNA bioapplications is hindered by a high vulnerability of exogeneous microRNA molecules to RNase degradation that occurs in extra- and intracellular physiological conditions. In this proof-of-concept study, we use a programmable self-assembled DNA nanostructure bearing a “shuriken” shape to not only deliver but more importantly protect a tumor suppressive microRNA-145 for a sufficiently long time to exert its therapeutic effect in human colorectal cancer cells. Our DNA nanostructure harbored complementary sequences that can hybridize with the microRNA cargo. This brings the microRNA–DNA duplex very close to the core structure such that the microRNA cargo becomes sterically shielded from RNase's degradative activity. Our novel DNA nanostructure based protector concept removes the degradative bottleneck that may plague other nucleic acid delivery strategies and presents a new paradigm towards exploiting these microRNAs for anti-cancer therapy.
ISSN: 2041-6520
DOI: 10.1039/C6SC01829G
Rights: © 2017 The Author(s) (published by Royal Society of Chemistry). This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles
SBS Journal Articles

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