dc.contributor.authorQian, H.
dc.contributor.authorTay, Chor Yong
dc.contributor.authorSetyawati, M. I.
dc.contributor.authorChia, S. L.
dc.contributor.authorLee, D. S.
dc.contributor.authorLeong, D. T.
dc.identifier.citationQian, H., Tay, C. Y., Setyawati, M. I., Chia, S. L., Lee, D. S., & Leong, D. T. (2017). Protecting microRNAs from RNase degradation with steric DNA nanostructures. Chemical Science, 8(2), 1062-1067.en_US
dc.description.abstractTumor suppressive microRNAs are potent molecules that might cure cancer, one day. Despite the many advanced strategies for delivery of these microRNAs to the cell, there are few therapeutic microRNAs in clinical use. Progress in microRNA bioapplications is hindered by a high vulnerability of exogeneous microRNA molecules to RNase degradation that occurs in extra- and intracellular physiological conditions. In this proof-of-concept study, we use a programmable self-assembled DNA nanostructure bearing a “shuriken” shape to not only deliver but more importantly protect a tumor suppressive microRNA-145 for a sufficiently long time to exert its therapeutic effect in human colorectal cancer cells. Our DNA nanostructure harbored complementary sequences that can hybridize with the microRNA cargo. This brings the microRNA–DNA duplex very close to the core structure such that the microRNA cargo becomes sterically shielded from RNase's degradative activity. Our novel DNA nanostructure based protector concept removes the degradative bottleneck that may plague other nucleic acid delivery strategies and presents a new paradigm towards exploiting these microRNAs for anti-cancer therapy.en_US
dc.format.extent6 p.en_US
dc.relation.ispartofseriesChemical Scienceen_US
dc.rights© 2017 The Author(s) (published by Royal Society of Chemistry). This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.en_US
dc.titleProtecting microRNAs from RNase degradation with steric DNA nanostructuresen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US

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