dc.contributor.authorYang, Yun
dc.contributor.authorWu, Yichao
dc.contributor.authorHu, Yidan
dc.contributor.authorWang, Hua
dc.contributor.authorGuo, Lin
dc.contributor.authorFredrickson, James K.
dc.contributor.authorCao, Bin
dc.contributor.editorAtomi, Haruyuki*
dc.date.accessioned2018-02-02T08:50:09Z
dc.date.available2018-02-02T08:50:09Z
dc.date.issued2017
dc.identifier.citationYang, Y., Wu, Y., Hu, Y., Wang, H., Guo, L., Fredrickson, J. K., et al. (2017). Harnessing the Periplasm of Bacterial Cells To Develop Biocatalysts for the Biosynthesis of Highly Pure Chemicals. Applied and Environmental Microbiology, 84(1), e01693-17-.en_US
dc.identifier.issn0099-2240en_US
dc.identifier.urihttp://hdl.handle.net/10220/44390
dc.description.abstractAlthough biocatalytic transformation has shown great promise in chemical synthesis, there remain significant challenges in controlling high selectivity without the formation of undesirable by-products. For instance, few attempts to construct biocatalysts for de novo synthesis of pure flavin mononucleotide (FMN) have been successful, due to riboflavin (RF) accumulating in the cytoplasm and being secreted with FMN. To address this problem, we show here a novel biosynthesis strategy, compartmentalizing the final FMN biosynthesis step in the periplasm of an engineered Escherichia coli strain. This construct is able to overproduce FMN with high specificity (92.4% of total excreted flavins). Such a biosynthesis approach allows isolation of the final biosynthesis step from the cytoplasm to eliminate undesirable by-products, providing a new route to develop biocatalysts for the synthesis of high-purity chemicals.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent11 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesApplied and Environmental Microbiologyen_US
dc.rights© 2017 American Society for Microbiology. This paper was published in Applied and Environmental Microbiology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The published version is available at: [http://dx.doi.org/10.1128/AEM.01693-17]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en_US
dc.subjectBiosynthesisen_US
dc.subjectPeriplasmen_US
dc.titleHarnessing the Periplasm of Bacterial Cells To Develop Biocatalysts for the Biosynthesis of Highly Pure Chemicalsen_US
dc.typeJournal Article
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.contributor.schoolSchool of Civil and Environmental Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1128/AEM.01693-17


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