High resolution and deep tissue imaging using a near infrared acoustic resolution photoacoustic microscopy
Date of Issue2018
Proceedings of SPIE - Photons Plus Ultrasound: Imaging and Sensing 2018
School of Chemical and Biomedical Engineering
It is always a great challenge for pure optical techniques to maintain good resolution and imaging depth at the same time. Photoacoustic imaging is an emerging technique which can overcome the limitation by pulsed light illumination and acoustic detection. Here, we report a Near Infrared Acoustic-Resolution Photoacoustic Microscopy (NIR-AR-PAM) systm with 30 MHz transducer and 1064 nm illumination which can achieve a lateral resolution of around 88 μm and imaging depth of 9.2 mm. Compared to visible light NIR beam can penetrate deeper in biological tissue due to weaker optical attenuation. In this work, we also demonstrated the in vivo imaging capabilty of NIRARPAM by near infrared detection of SLN with black ink as exogenous photoacoustic contrast agent in a rodent model.
Acoustic Resolution Photoacoustic Microscopy
© 2018 Society of Photo-optical Instrumentation Engineers (SPIE). This paper was published in Proceedings of SPIE - Photons Plus Ultrasound: Imaging and Sensing 2018 and is made available as an electronic reprint (preprint) with permission of SPIE. The published version is available at: [http://dx.doi.org/10.1117/12.2287838]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.