Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications
Upputuri, Paul Kumar
Date of Issue2018
Proceedings of SPIE - Quantitative Phase Imaging IV
School of Chemical and Biomedical Engineering
Phase shifting white light interferometry (PSWLI) has been widely used for optical metrology applications because of their precision, reliability, and versatility. White light interferometry using monochrome CCD makes the measurement process slow for metrology applications. WLI integrated with Red-Green-Blue (RGB) CCD camera is finding imaging applications in the fields optical metrology and bio-imaging. Wavelength dependent refractive index profiles of biological samples were computed from colour white light interferograms. In recent years, whole-filed refractive index profiles of red blood cells (RBCs), onion skin, fish cornea, etc. were measured from RGB interferograms. In this paper, we discuss the bio-imaging applications of colour CCD based white light interferometry. The approach makes the measurement faster, easier, cost-effective, and even dynamic by using single fringe analysis methods, for industrial applications.
Phase Shifting Interferometry
© 2018 Society of Photo-optical Instrumentation Engineers (SPIE). This paper was published in Proceedings of SPIE - Quantitative Phase Imaging IV and is made available as an electronic reprint (preprint) with permission of SPIE. The published version is available at: [http://dx.doi.org/10.1117/12.2287090]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.