Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87606
Title: A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting
Authors: Shi, Pujiang
Tan, Yong Sheng Edgar
Yeong, Wai Yee
Li, Hoi Yeung
Laude, Augustinus
Keywords: Bioprinting
Retina
Issue Date: 2018
Source: Shi, P., Tan, Y. S. E., Yeong, W. Y., Li, H. Y., & Laude, A. (2018). A bilayer photoreceptor-retinal tissue model with gradient cell density design: a study of microvalve-based bioprinting. Journal of Tissue Engineering and Regenerative Medicine, 12(5), 1297-1306.
Series/Report no.: Journal of Tissue Engineering and Regenerative Medicine
Abstract: ARPE‐19 and Y79 cells were precisely and effectively delivered to form an in vitro retinal tissue model via 3D cell bioprinting technology. The samples were characterized by cell viability assay, hematoxylin and eosin (HE) and immunofluorescent staining, scanning electrical microscopy (SEM) and confocal microscopy etc. The bioprinted ARPE‐19 cells formed a high‐quality cell monolayer in 14 days. Manually seeded ARPE‐19 cells were poorly controlled during and after cell seeding, and they aggregated to form uneven cell layer. The Y79 cells were subsequently bioprinted on the ARPE‐19 cell monolayer to form two distinctive patterns. The microvalve‐based bioprinting is efficient and accurate to build the in vitro tissue models with the potential to provide similar pathological responses and mechanism to human diseases, to mimic the phenotypic endpoints that are comparable to clinical studies, and to provide a realistic prediction of clinical efficacy.
URI: https://hdl.handle.net/10356/87606
http://hdl.handle.net/10220/44606
ISSN: 1932-6254
DOI: http://dx.doi.org/10.1002/term.2661
Rights: © 2018 John Wiley & Sons Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/term.2661].
metadata.item.grantfulltext: open
metadata.item.fulltext: With Fulltext
Appears in Collections:MAE Journal Articles
SBS Journal Articles
SC3DP Journal Articles

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.