A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
Chandy, Kanianthara George
Date of Issue2017
Lee Kong Chian School of Medicine (LKCMedicine)
Traditionally, ocular surface cytology is studied with techniques such as spatula technology and brush technology. The problem with these techniques is that they may induce traumatic lesions on the surface of the eye, which can progress to scarring, eyelid deformity, limbal stem cell deficiency and in some cases, cause great discomfort to the subject. To avoid these clinical problems, impression cytology (IC) was developed to diagnose dry eye disease and later neoplasia, atopic disease, vernal keratoconjunctivitis and keratoconjunctivitis sicca. Typically, clinicians manually cut filter papers into required shapes and apply these to the ocular surface. Here, we describe how to perform IC using a commercially available medical device. This technique is explained here followed by immunophenotyping by flow cytometry. This technique requires less manual handling and causes less injury to the ocular surface.
Journal of Visualized Experiments
© 2017 The Author(s) (Journal of Visualized Experiments). This paper was published under Creative Commons Attribution-Non Commercial-No Derivs 3.0 Unported Agreement in Journal of Visualized Experiments and is made available as an electronic reprint (preprint) with permission of The Author(s) (Journal of Visualized Experiments). The published version is available at: [http://dx.doi.org/10.3791/55591]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.