dc.contributor.authorConic, Sascha
dc.contributor.authorDesplancq, Dominique
dc.contributor.authorFerrand, Alexia
dc.contributor.authorFischer, Veronique
dc.contributor.authorHeyer, Vincent
dc.contributor.authorReina San Martin, Bernardo
dc.contributor.authorPontabry, Julien
dc.contributor.authorOulad-Abdelghani, Mustapha
dc.contributor.authorBabu N., Kishore
dc.contributor.authorWright, Graham D.
dc.contributor.authorMolina, Nacho
dc.contributor.authorWeiss, Etienne
dc.contributor.authorTora, László
dc.identifier.citationConic, S., Desplancq, D., Ferrand, A., Fischer, V., Heyer, V., Reina San Martin, B., et al. (2018). Imaging of native transcription factors and histone phosphorylation at high resolution in live cells. Journal of Cell Biology, 217(4), 1537-1552.en_US
dc.description.abstractFluorescent labeling of endogenous proteins for live-cell imaging without exogenous expression of tagged proteins or genetic manipulations has not been routinely possible. We describe a simple versatile antibody-based imaging approach (VANIMA) for the precise localization and tracking of endogenous nuclear factors. Our protocol can be implemented in every laboratory allowing the efficient and nonharmful delivery of organic dye-conjugated antibodies, or antibody fragments, into different metazoan cell types. Live-cell imaging permits following the labeled probes bound to their endogenous targets. By using conventional and super-resolution imaging we show dynamic changes in the distribution of several nuclear transcription factors (i.e., RNA polymerase II or TAF10), and specific phosphorylated histones (γH2AX), upon distinct biological stimuli at the nanometer scale. Hence, considering the large panel of available antibodies and the simplicity of their implementation, VANIMA can be used to uncover novel biological information based on the dynamic behavior of transcription factors or posttranslational modifications in the nucleus of single live cells.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent16 p.en_US
dc.relation.ispartofseriesJournal of Cell Biologyen_US
dc.rights© 2018 Conic et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).en_US
dc.titleImaging of native transcription factors and histone phosphorylation at high resolution in live cellsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US

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