Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
Amrutkar, Suyog Madhav
Date of Issue2018
School of Physical and Mathematical Sciences
We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment and pull down assays.
© 2018 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioconjugate Chemistry, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acs.bioconjchem.7b00826].