Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83167
Title: Block copolymer nanoparticles remove biofilms of drug-resistant gram-positive bacteria by nanoscale bacterial debridement
Authors: Li, Jianghua
Zhang, Kaixi
Ruan, Lin
Chin, Seow Fong
Wickramasinghe, Nirmani
Liu, Hanbin
Ravikumar, Vikashini
Ren, Jinghua
Duan, Hongwei
Yang, Liang
Chan-Park, Mary Bee Eng
Keywords: Antibiofilm
Biocompatibility
Issue Date: 2018
Source: Li, J., Zhang, K., Ruan, L., Chin, S. F., Wickramasinghe, N., Liu, H., et al. (2018). Block Copolymer Nanoparticles Remove Biofilms of Drug-Resistant Gram-Positive Bacteria by Nanoscale Bacterial Debridement. Nano Letters.
Series/Report no.: Nano Letters
Abstract: Biofilms and the rapid evolution of multidrug resistance complicate the treatment of bacterial infections. Antibiofilm agents such as metallic–inorganic nanoparticles or peptides act by exerting antibacterial effects and, hence, do not combat biofilms of antibiotics-resistant strains. In this Letter, we show that the block copolymer DA95B5, dextran-block-poly((3-acrylamidopropyl) trimethylammonium chloride (AMPTMA)-co-butyl methacrylate (BMA)), effectively removes preformed biofilms of various clinically relevant multidrug-resistant Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococci (VRE V583), and Enteroccocus faecalis (OG1RF). DA95B5 self-assembles into core–shell nanoparticles with a nonfouling dextran shell and a cationic core. These nanoparticles diffuse into biofilms and attach to bacteria but do not kill them; instead, they promote the gradual dispersal of biofilm bacteria, probably because the solubility of the bacteria–nanoparticle complex is enhanced by the nanoparticle dextran shell. DA95B5, when applied as a solution to a hydrogel pad dressing, shows excellent in vivo MRSA biofilm removal efficacy of 3.6 log reduction in a murine excisional wound model, which is significantly superior to that for vancomycin. Furthermore, DA95B5 has very low in vitro hemolysis and negligible in vivo acute toxicity. This new strategy for biofilm removal (nanoscale bacterial debridement) is orthogonal to conventional rapidly developing resistance traits in bacteria so that it is as effective toward resistant strains as it is toward sensitive strains and may have widespread applications.
URI: https://hdl.handle.net/10356/83167
http://hdl.handle.net/10220/45046
ISSN: 1530-6984
DOI: 10.1021/acs.nanolett.8b01000
Rights: © 2018 American Chemical Society (ACS). This is the author created version of a work that has been peer reviewed and accepted for publication by Nano Letters, American Chemical Society (ACS). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acs.nanolett.8b01000].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SCBE Journal Articles
SCELSE Journal Articles

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