Separation of pathogenic bacteria by chain length
Author
Beech, Jason P.
Ho, Bao Dang
Garriss, Geneviève
Oliveira, Vitor
Henriques-Normark, Birgitta
Tegenfeldt, Jonas O.
Date of Issue
2018School
Lee Kong Chian School of Medicine (LKCMedicine)
Related Organization
Singapore Centre for Environmental Life Sciences Engineering
Version
Published version
Abstract
Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separatesubpopulations of bacteria depending on known properties that affect their capability to cau se disease(virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease.Separation of cultured pneumococci into subpopulations based on morphological type (single cocci,diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, wepresent separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains in our device.
Subject
Deterministic Lateral Displacement
Separation
Separation
Type
Journal Article
Series/Journal Title
Analytica Chimica Acta
Rights
© 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Collections
http://dx.doi.org/10.1016/j.aca.2017.11.050
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