Separation of pathogenic bacteria by chain length
Beech, Jason P.
Ho, Bao Dang
Tegenfeldt, Jonas O.
Date of Issue2018
Lee Kong Chian School of Medicine
Singapore Centre for Environmental Life Sciences and Engineering
Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separatesubpopulations of bacteria depending on known properties that affect their capability to cau se disease(virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease.Separation of cultured pneumococci into subpopulations based on morphological type (single cocci,diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, wepresent separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains in our device.
Deterministic Lateral Displacement
Analytica Chimica Acta
© 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).