dc.contributor.authorSheraton, Muniraj Vivek
dc.contributor.authorYam, Joey Kuok Hoong
dc.contributor.authorTan, Chuan Hao
dc.contributor.authorOh, H. S.
dc.contributor.authorMancini, E.
dc.contributor.authorYang, Liang
dc.contributor.authorRice, Scott A.
dc.contributor.authorSloot, Peter M. A.
dc.identifier.citationSheraton, M. V., Yam, J. K. H., Tan, C. H., Oh, H. S., Mancini, E., Yang, L. & et al. (2018). Mesoscopic Energy Minimization Drives Pseudomonas aeruginosa Biofilm Morphologies and Consequent Stratification of Antibiotic Activity Based on Cell Metabolism. Antimicrobial Agents and Chemotherapy, 62(5), e02544-17-.en_US
dc.description.abstractSegregation of bacteria based on their metabolic activities in biofilms plays an important role in the development of antibiotic resistance. Mushroom-shaped biofilm structures, which are reported for many bacteria, exhibit topographically varying levels of multiple drug resistance from the cap of the mushroom to its stalk. Understanding the dynamics behind the formation of such structures can aid in design of drug delivery systems, antibiotics, or physical systems for removal of biofilms. We explored the development of metabolically heterogeneous Pseudomonas aeruginosa biofilms using numerical models and laboratory knockout experiments on wild-type and chemotaxis-deficient mutants. We show that chemotactic processes dominate the transformation of slender and hemispherical structures into mushroom structures with a signature cap. Cellular Potts model simulation and experimental data provide evidence that accelerated movement of bacteria along the periphery of the biofilm, due to nutrient cues, results in the formation of mushroom structures and bacterial segregation. Multidrug resistance of bacteria is one of the most threatening dangers to public health. Understanding the mechanisms of the development of mushroom-shaped biofilms helps to identify the multidrug-resistant regions. We decoded the dynamics of the structural evolution of bacterial biofilms and the physics behind the formation of biofilm structures as well as the biological triggers that produce them. Combining in vitro gene knockout experiments with in silico models showed that chemotactic motility is one of the main driving forces for the formation of stalks and caps. Our results provide physicists and biologists with a new perspective on biofilm removal and eradication strategies.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent11 p.en_US
dc.relation.ispartofseriesAntimicrobial Agents and Chemotherapyen_US
dc.rights© 2018 Sheraton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.subjectCellular Potts Modelen_US
dc.subjectMushroom-shaped Biofilmen_US
dc.titleMesoscopic energy minimization drives pseudomonas aeruginosa biofilm morphologies and consequent stratification of antibiotic activity based on cell metabolismen_US
dc.typeJournal Article
dc.contributor.researchComplexity Instituteen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolInterdisciplinary Graduate School (IGS)en_US
dc.description.versionPublished versionen_US
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen_US

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