dc.contributor.authorShi, Pujiang
dc.contributor.authorTan, Edgar Yong Sheng
dc.contributor.authorYeong, Wai Yee
dc.contributor.authorLaude, Augustinus
dc.date.accessioned2018-07-20T02:47:54Z
dc.date.available2018-07-20T02:47:54Z
dc.date.issued2017
dc.identifier.citationShi, P., Tan, E. Y. S., Yeong, W. Y., & Laude, A. (2017). Hybrid three-dimensional (3D) bioprinting of retina equivalent for ocular research. International Journal of Bioprinting, 3(2), 138-146.en_US
dc.identifier.urihttp://hdl.handle.net/10220/45150
dc.description.abstractIn this article, a hybrid retina construct was created via three-dimensional (3D) bioprinting technology. The construct was composed of a PCL ultrathin membrane, ARPE-19 cell monolayer and Y79 cell-laden alginate/pluronic bioink. 3D bioprinting technology was applied herein to deliver the ARPE-19 cells and Y79 cell-laden bioink to ensure homogeneous ARPE-19 cell seeding; subsequently, two distinctive Y79 cell-seeding patterns were bioprinted on top of the ARPE-19 cell monolayer. The bioprinted ARPE-19 cells were evaluated by prestoblue assay, F-actin, and hematoxylin/eosin (HE) staining, and then the cells were observed under laser scanning and invert microscopy for 14 days. The Y79 cells in alginate/pluronic bioink after bioprinting had been closely monitored for 7 days. Live/dead assay and scanning electrical microscopy (SEM) were employed to investigate Y79 cell viability and morphology. Both the ARPE-19 and Y79 cells were in excellent condition, and the successfully bioprinted retina model could be utilized in drug delivery, disease mechanism and treatment method discoveries.en_US
dc.format.extent9 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesInternational Journal of Bioprintingen_US
dc.rights© 2017 The Author(s) (International Journal of Bioprinting) (published by Whioce Publishing Pte Ltd). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectRetinaen_US
dc.subjectBioprintingen_US
dc.titleHybrid three-dimensional (3D) bioprinting of retina equivalent for ocular researchen_US
dc.typeJournal Article
dc.contributor.researchSingapore Centre for 3D Printingen_US
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.18063/IJB.2017.02.008
dc.description.versionPublished versionen_US


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