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|Title:||Probing the internal micromechanical properties of Pseudomonas aeruginosa biofilms by Brillouin imaging||Authors:||Rice, Scott A,
Song, C. Z.
Allsopp, L. P.
|Issue Date:||2017||Source:||Karampatzakis, A., Song, C. Z., Allsopp, L. P., Filloux, A., Rice, S. A., Cohen, Y., et al. (2017). Probing the internal micromechanical properties of Pseudomonas aeruginosa biofilms by Brillouin imaging. npj Biofilms and Microbiomes, 3(1), 20-.||Series/Report no.:||npj Biofilms and Microbiomes||Abstract:||Biofilms are organised aggregates of bacteria that adhere to each other or surfaces. The matrix of extracellular polymeric substances that holds the cells together provides the mechanical stability of the biofilm. In this study, we have applied Brillouin microscopy, a technique that is capable of measuring mechanical properties of specimens on a micrometre scale based on the shift in frequency of light incident upon a sample due to thermal fluctuations, to investigate the micromechanical properties of an active, live Pseudomonas aeruginosa biofilm. Using this non-contact and label-free technique, we have extracted information about the internal stiffness of biofilms under continuous flow. No correlation with colony size was found when comparing the averages of Brillouin shifts of two-dimensional cross-sections of randomly selected colonies. However, when focusing on single colonies, we observed two distinct spatial patterns: in smaller colonies, stiffness increased towards their interior, indicating a more compact structure of the centre of the colony, whereas, larger (over 45 μm) colonies were found to have less stiff interiors.||URI:||https://hdl.handle.net/10356/86649
|DOI:||10.1038/s41522-017-0028-z||Rights:||© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
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