dc.contributor.authorKarampatzakis, A.
dc.contributor.authorSong, C. Z.
dc.contributor.authorAllsopp, L. P.
dc.contributor.authorFilloux, A.
dc.contributor.authorRice, Scott A,
dc.contributor.authorCohen, Yehuda
dc.contributor.authorWohland, T.
dc.contributor.authorTörök, P.
dc.date.accessioned2018-07-30T02:14:42Z
dc.date.available2018-07-30T02:14:42Z
dc.date.issued2017
dc.identifier.citationKarampatzakis, A., Song, C. Z., Allsopp, L. P., Filloux, A., Rice, S. A., Cohen, Y., et al. (2017). Probing the internal micromechanical properties of Pseudomonas aeruginosa biofilms by Brillouin imaging. npj Biofilms and Microbiomes, 3(1), 20-.en_US
dc.identifier.urihttp://hdl.handle.net/10220/45340
dc.description.abstractBiofilms are organised aggregates of bacteria that adhere to each other or surfaces. The matrix of extracellular polymeric substances that holds the cells together provides the mechanical stability of the biofilm. In this study, we have applied Brillouin microscopy, a technique that is capable of measuring mechanical properties of specimens on a micrometre scale based on the shift in frequency of light incident upon a sample due to thermal fluctuations, to investigate the micromechanical properties of an active, live Pseudomonas aeruginosa biofilm. Using this non-contact and label-free technique, we have extracted information about the internal stiffness of biofilms under continuous flow. No correlation with colony size was found when comparing the averages of Brillouin shifts of two-dimensional cross-sections of randomly selected colonies. However, when focusing on single colonies, we observed two distinct spatial patterns: in smaller colonies, stiffness increased towards their interior, indicating a more compact structure of the centre of the colony, whereas, larger (over 45 μm) colonies were found to have less stiff interiors.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent7 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesnpj Biofilms and Microbiomesen_US
dc.rights© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectBrillouin Imagingen_US
dc.subjectBiofilmsen_US
dc.titleProbing the internal micromechanical properties of Pseudomonas aeruginosa biofilms by Brillouin imagingen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1038/s41522-017-0028-z
dc.description.versionPublished versionen_US
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen_US


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