Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87589
Title: Rapid cell separation with minimal manipulation for autologous cell therapies
Authors: Smith, Alban J.
O’Rorke, Richard D.
Kale, Akshay
Rimsa, Roberts
Tomlinson, Matthew J.
Kirkham, Jennifer
Davies, A. Giles
Wälti, Christoph
Wood, Christopher D.
Keywords: Minimal Manipulation
Autologous Cell Therapies
Issue Date: 2017
Source: Smith, A. J., O’Rorke, R. D., Kale, A., Rimsa, R., Tomlinson, M. J., Kirkham, J., et al. (2017). Rapid cell separation with minimal manipulation for autologous cell therapies. Scientific Reports, 7, 41872-.
Series/Report no.: Scientific Reports
Abstract: The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling. Thus a rapid, label-free separation technology that does not affect cell functionality, viability or phenotype is highly desirable. Here, we demonstrate separation of viable from non-viable human stromal cells using remote dielectrophoresis, in which an electric field is coupled into a microfluidic channel using shear-horizontal surface acoustic waves, producing an array of virtual electrodes within the channel. This allows high-throughput dielectrophoretic cell separation in high conductivity, physiological-like fluids, overcoming the limitations of conventional dielectrophoresis. We demonstrate viable/non-viable separation efficacy of >98% in pre-purified mesenchymal stromal cells, extracted from human dental pulp, with no adverse effects on cell viability, or on their subsequent osteogenic capabilities.
URI: https://hdl.handle.net/10356/87589
http://hdl.handle.net/10220/45460
ISSN: 2045-2322
DOI: 10.1038/srep41872
Rights: © 2017 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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