dc.contributor.authorZhu, Xing
dc.contributor.authorHe, Bin
dc.contributor.authorZhao, Changwen
dc.contributor.authorMa, Yuhong
dc.contributor.authorYang, Wantai
dc.date.accessioned2018-08-06T07:50:33Z
dc.date.available2018-08-06T07:50:33Z
dc.date.issued2018
dc.identifier.citationZhu, X., He, B., Zhao, C., Ma, Y., & Yang, W. (2018). Simultaneously and separately immobilizing incompatible dual-enzymes on polymer substrate via visible light induced graft polymerization. Applied Surface Science, 436, 73-79.en_US
dc.identifier.issn0169-4332en_US
dc.identifier.urihttp://hdl.handle.net/10220/45480
dc.description.abstractDeveloping facile and mild strategy to construct multi-enzymes immobilization system has attracted considerable attentions in recent years. Here a simple immobilization strategy called visible light induced graft polymerization that can simultaneously and separately encapsulate two kinds of enzymes on one polymer film was proposed. Two incompatible enzymes, trypsin and transglutaminase (TGase) were selected as model dual-enzymes system and simultaneously immobilized on two sides of low-density polyethylene (LDPE) film. After immobilization, it was found that more than 90% of the enzymes can be embedded into dual-enzymes loaded film without leakage. And the activities of both separately immobilized enzymes were higher than the activities of mixed co-immobilized enzymes or the sequential immobilized ones. This dual-enzymes loaded film (DEL film) showed excellent recyclability and can retain >87% activities of both enzymes after 4 cycles of utilization. As an example, this DEL film was used to conjugate a prodrug of cytarabine with a target peptide. The successful preparation of expected product demonstrated that the separately immobilized two enzymes can worked well together to catalyze a two-step reaction.en_US
dc.format.extent40 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesApplied Surface Scienceen_US
dc.rights© 2017 Elsevier B.V. This is the author created version of a work that has been peer reviewed and accepted for publication by Applied Surface Science, Elsevier B.V. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.apsusc.2017.11.284].en_US
dc.subjectVisible Lighten_US
dc.subjectGraft Polymerizationen_US
dc.titleSimultaneously and separately immobilizing incompatible dual-enzymes on polymer substrate via visible light induced graft polymerizationen_US
dc.typeJournal Article
dc.contributor.researchNanyang Environment and Water Research Instituteen_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.apsusc.2017.11.284
dc.description.versionAccepted versionen_US


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