Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87804
Title: Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound
Authors: Teo, Ailing
Morshedi, Amir
Wang, Jen-Chieh
Zhou, Yufeng
Lim, Mayasari
Keywords: Embryonic Stem Cells
Cardiomyogenesis
Issue Date: 2017
Source: Teo, A., Morshedi, A., Wang, J. C., Zhou, Y., & Lim, M. (2017). Enhancement of cardiomyogenesis in murine stem cells by low-intensity ultrasound. Journal of Ultrasound in Medicine, 36(8), 1693-1706.
Series/Report no.: Journal of Ultrasound in Medicine
Abstract: Objectives: Low‐intensity ultrasound (LIUS) has been shown to enhance bone and cartilage regeneration from stem cells. The ease of its incorporation makes it an attractive mechanical stimulus for not only osteogenesis and chondrogenesis, but also cardiomyogenesis. However, to date, no study has investigated its effects on cardiomyogenesis from embryonic stem cells. Methods: In this study, murine embryonic stem cells were differentiated via embryoid body formation and plating, and after 3 days they were subjected to daily 10 minutes of LIUS treatment with various conditions: (1) low‐pulsed (21 mW/cm2, 20% duty cycle), (2) low‐continuous, (3) high‐pulsed (147 mW/cm2, 20% duty cycle), and (4) high‐continuous LIUS. Results: Low‐pulsed and high‐continuous LIUS had improved beating rates of contractile areas as well as increased late cardiac gene expressions, such as α‐ and β‐myosin heavy chain and cardiac troponin T, showing its benefits on cardiomyocyte differentiation. Meanwhile, an early endodermal marker, α‐fetoprotein, was significantly attenuated after LIUS treatments. Conclusions: With these observations, it is demonstrated that LIUS simulation could enhance cardiomyogenesis from embryonic stem cells and increase its selectivity toward cardiomyocytes by reducing spontaneous differentiation.
URI: https://hdl.handle.net/10356/87804
http://hdl.handle.net/10220/45527
ISSN: 0278-4297
DOI: http://dx.doi.org/10.7863/ultra.16.12042
Rights: © 2017 American Institute of Ultrasound in Medicine. This paper was published in Journal of Ultrasound in Medicine and is made available as an electronic reprint (preprint) with permission of American Institute of Ultrasound in Medicine. The published version is available at: [http://dx.doi.org/10.7863/ultra.16.12042]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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