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|Title:||Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs||Authors:||Reid, David W.
|Issue Date:||2017||Source:||Reid, D. W., Xu, D., Chen, P., Yang, H., & Sun, L. (2017). Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs. Scientific Reports, 7(1), 5681-.||Series/Report no.:||Scientific Reports||Abstract:||The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at the level of translational regulation remains poorly understood. To systemically determine the translational control of gene expression in adipose tissue, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue. The most prominent layer of translational regulation was the increased translation efficiency of genes encoding mitochondria components in brown adipocytes relative to white. Systemic analysis of the regulatory interactions between microRNAs and their targets revealed that microRNAs were more active in repressing targets’ mRNA abundance and translation in brown fat. Together, our data comprehensively delineated a landscape integrating transcriptome and translatome in adipose tissue.||URI:||https://hdl.handle.net/10356/87975
|ISSN:||2045-2322||DOI:||http://dx.doi.org/10.1038/s41598-017-06077-3||Rights:||© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.||metadata.item.grantfulltext:||open||metadata.item.fulltext:||With Fulltext|
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