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|Title:||Lybatides from Lycium barbarum contain an unusual cystine-stapled helical peptide scaffold||Authors:||Tan, Wei Liang
Wong, Ka Ho
Tan, Hong Wei
Tam, James P.
|Issue Date:||2017||Source:||Tan, W. L., Wong, K. H., Lei, J., Sakai, N., Tan, H. W., Hilgenfeld, R., & Tam, J. P. (2017). Lybatides from Lycium barbarum contain an unusual cystine-stapled helical peptide scaffold. Scientific Reports, 7, 5194-. doi:10.1038/s41598-017-05037-1||Series/Report no.:||Scientific Reports||Abstract:||Cysteine-rich peptides (CRPs) of 2–6 kDa are generally thermally and proteolytically stable because of their multiple cross-bracing disulfide bonds. Here, we report the discovery and characterization of two novel cystine-stapled CRPs, designated lybatide 1 and 2 (lyba1 and lyba2), from the cortex of Lycium barbarum root. Lybatides, 32 to 33 amino acids in length, are hyperstable and display a novel disulfide connectivity with a cysteine motif of C-C-C-C-CC-CC which contains two pairs of adjacent cysteines (-CC-CC). X-ray structure analysis revealed the presence of a single cystine-stabilized (α + π)-helix in lyba2, a rare feature of CRPs. Together, our results suggest that lybatides, one of the smallest four-disulfide-constrained plant CRPs, is a new family of CRPs. Additionally, this study provides new insights into the molecular diversity of plant cysteine-rich peptides and the unusual lybatide scaffold could be developed as a useful template for peptide engineering and therapeutic development.||URI:||https://hdl.handle.net/10356/88139
|ISSN:||2045-2322||DOI:||http://dx.doi.org/10.1038/s41598-017-05037-1||Rights:||© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
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