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|Title:||Extracellular vesicles and their detection with plasmonic bionsensors for the diagnosis of ovarian cancer||Authors:||Reiner, Agnes||Keywords:||DRNTU::Science::Medicine::Biosensors||Issue Date:||23-Aug-2018||Source:||Reiner, A. (2018). Extracellular vesicles and their detection with plasmonic bionsensors for the diagnosis of ovarian cancer. Doctoral thesis, Nanyang Technological University, Singapore.||Abstract:||Ovarian cancer is the deadliest gynecological cancer, due to its late diagnosis resulting in unsuccessful treatment. An earlier diagnosis at a stage, when the disease is still treatable, would improve the chances of survival immensely. To achieve such an early diagnosis extracellular vesicles (EVs) have been proposed as a new class of biomarkers, but the currently used methods for their isolation and analysis lack in specificity and sensitivity. Hence this project aimed to identify new EV-based biomarkers for an improved diagnosis of ovarian cancer by usage of lipid-binding proteins for EV isolation and to develop new highly sensitive biosensors for EV detection based on surface plasmon resonance (SPR). We achieved our aim by identifying a subpopulation of EVs that carries a distinct lipid and protein composition as candidate biomarker for ovarian cancer and will validate its value for clinical application in subsequent studies. Furthermore, we developed a plasmonic biosensor platform that was capable of detecting trace amounts of EVs by making use of magnetic nanoparticles for signal enhancement. The specific detection of cancer-derived EVs with this sensing platform will be studied in future.||URI:||https://hdl.handle.net/10356/88119
|DOI:||https://doi.org/10.32657/10220/45648||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||IGS Theses|
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