Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88280
Title: Single-cell transcriptomics of East-Asian pancreatic islets cells
Authors: Tay, Vanessa Shi Yun
Boehm, Bernhard Otto
Dorajoo, Rajkumar
Ali, Yusuf
Kang, Jonathan
Samydurai, Sudhagar
Liu, Jianjun
Keywords: Gene Expression
Islet Cell
DRNTU::Science::Biological sciences
Issue Date: 2017
Source: Dorajoo, R., Ali, Y., Tay, V. S. Y., Kang, J., Samydurai, S., Liu, J., & Boehm, B. O. (2017). Single-cell transcriptomics of East-Asian pancreatic islets cells. Scientific Reports, 7, 5024-. doi:10.1038/s41598-017-05266-4
Series/Report no.: Scientific Reports
Abstract: Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between α- and β-cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 α, 105 β, 6 δ endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between α- and β-cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian β-cells. We report on East-Asian α- and β-cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian β-cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis.
URI: https://hdl.handle.net/10356/88280
http://hdl.handle.net/10220/45671
ISSN: 2045-2322
DOI: 10.1038/s41598-017-05266-4
Rights: © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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