dc.contributor.authorSherchan, Samendra
dc.contributor.authorMiles, Syreeta
dc.contributor.authorIkner, Luisa
dc.contributor.authorYu, Hye-Weon
dc.contributor.authorSnyder, Shane Allen
dc.contributor.authorPepper, Ian L.
dc.identifier.citationSherchan, S., Miles, S., Ikner, L., Yu, H.-W., Snyder, S. A., & Pepper, I. L. (2018). Near real-time detection of E. coli in reclaimed water. Sensors, 18(7), 2303-. doi:10.3390/s18072303en_US
dc.description.abstractAdvanced treatment of reclaimed water prior to potable reuse normally results in the inactivation of bacterial populations, however, incremental treatment failure can result in bacteria, including pathogens, remaining viable. Therefore, potential microorganisms need to be detected in real-time to preclude potential adverse human health effects. Real-time detection of microbes presents unique problems which are dependent on the water quality of the test water, including parameters such as particulate content and turbidity, and natural organic matter content. In addition, microbes are unusual in that: (i) viability and culturability are not always synonymous; (ii) viability in water can be reduced by osmotic stress; and (iii) bacteria can invoke repair mechanisms in response to UV disinfection resulting in regrowth of bacterial populations. All these issues related to bacteria affect the efficacy of real-time detection for bacteria. Here we evaluate three different sensors suitable for specific water qualities. The sensor A is an on-line, real-time sensor that allows for the continuous monitoring of particulates (including microbial contaminants) using multi-angle-light scattering (MALS) technology. The sensor B is a microbial detection system that uses optical technique, Mie light scattering, for particle sizing and fluorescence emission for viable bacteria detection. The last sensor C was based on adenosine triphosphate (ATP) production. E. coli was used a model organism and out of all tested sensors, we found the sensor C to be the most accurate. It has a great potential as a surrogate parameter for microbial loads in test waters and be useful for process control in treatment trains.en_US
dc.format.extent10 p.en_US
dc.rights© 2018 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en_US
dc.subjectWater Qualityen_US
dc.subjectOnline Sensorsen_US
dc.subjectDRNTU::Engineering::Environmental engineeringen_US
dc.titleNear real-time detection of E. coli in reclaimed wateren_US
dc.typeJournal Article
dc.contributor.researchNanyang Environment and Water Research Instituteen_US
dc.description.versionPublished versionen_US

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