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|Title:||Structure and dynamics in the nucleosome revealed by solid-state NMR||Authors:||Shi, Xiangyan
|Issue Date:||2018||Source:||Shi, X., Prasanna, C., Nagashima, T., Yamazaki, T., Pervushin, K.,& Nordenskiöld, L. (2018). Structure and dynamics in the nucleosome revealed by solid-state NMR. Angewandte Chemie International Edition, 57(31), 9734-9738. doi:10.1002/anie.201804707||Series/Report no.:||Angewandte Chemie International Edition||Abstract:||Eukaryotic chromatin structure and dynamics play key roles in genomic regulation. In the current study, we implemented solid-state NMR (SSNMR) technique to investigate secondary structure and intramolecular dynamics of the nucleosome core particle (NCP) and nucleosome array at atomic level. Secondary structure elements are successfully localized in the human histone H4 in the NCP precipitated with Mg2+. In particular, dynamics on nanosecond to microsecond and microsecond to millisecond timescales are elucidated, revealing diverse internal motions in the histone H4 protein. The results suggest that the histone dynamics might contribute to the regulation of chromatin mobility and DNA accessibility. Furthermore, our study reveals that H4 in the nucleosome array adopts the same structure and show similar internal dynamics as that in the NCP assembly while exhibiting relatively restricted motions in several regions consisting of residues in the N-terminus, Loop 1 and the α3 helix region.||URI:||https://hdl.handle.net/10356/88142
|ISSN:||1433-7851||DOI:||http://dx.doi.org/10.1002/anie.201804707||Rights:||© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Angewandte Chemie International Edition, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/anie.201804707].||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
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