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Title: The anti-oxidant defense system of the marine polar ciliate euplotes nobilii : characterization of the msrb gene family
Authors: Ricci, Francesca
Bakiu, Rigers
Santovito, Gianfranco
Lauro, Federico M.
Grzymski, Joseph J.
Read, Robert
Luporini, Pierangelo
Vallesi, Adriana
Keywords: MsrB Proteins
Oxidative Stress
Issue Date: 2017
Source: Ricci, F., Lauro, F. M., Grzymski, J. J., Read, R., Bakiu, R., Santovito, G., ...Vallesi, A. (2017). The anti-oxidant defense system of the marine polar ciliate euplotes nobilii: characterization of the msrB gene family. Biology, 6(1), 4-. doi:10.3390/biology6010004
Series/Report no.: Biology
Abstract: Organisms living in polar waters must cope with an extremely stressful environment dominated by freezing temperatures, high oxygen concentrations and UV radiation. To shed light on the genetic mechanisms on which the polar marine ciliate, Euplotes nobilii, relies to effectively cope with the oxidative stress, attention was focused on methionine sulfoxide reductases which repair proteins with oxidized methionines. A family of four structurally distinct MsrB genes, encoding enzymes specific for the reduction of the methionine-sulfoxide R-forms, were identified from a draft of the E. nobilii transcriptionally active (macronuclear) genome. The En-MsrB genes are constitutively expressed to synthesize proteins markedly different in amino acid sequence, number of CXXC motifs for zinc-ion binding, and presence/absence of a cysteine residue specific for the mechanism of enzyme regeneration. The En-MsrB proteins take different localizations in the nucleus, mitochondria, cytosol and endoplasmic reticulum, ensuring a pervasive protection of all the major subcellular compartments from the oxidative damage. These observations have suggested to regard the En-MsrB gene activity as playing a central role in the genetic mechanism that enables E. nobilii and ciliates in general to live in the polar environment.
ISSN: 2079-7737
DOI: 10.3390/biology6010004
Rights: © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
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