Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88962
Title: Mechanistic insights into PTS2-mediated peroxisomal protein import : the co-receptor PEX5L drastically increases the interaction strength between the cargo protein and the receptor PEX7
Authors: Kunze, Markus
Wiesinger, Christoph
Schmid, Johannes A.
Berger, Johannes
Malkani, Naila
Maurer-Stroh, Sebastian
Keywords: DRNTU::Science::Biological sciences
Conformational Change
Peroxisome
Issue Date: 2014
Source: Kunze, M., Malkani, N., Maurer-Stroh, S., Wiesinger, C., Schmid, J. A., & Berger, J. (2015). Mechanistic insights into PTS2-mediated peroxisomal protein Import : the co-receptor PEX5L drastically increases the interaction strength between the cargo protein and the receptor PEX7. Journal of Biological Chemistry, 290(8), 4928-4940. doi:10.1074/jbc.M114.601575
Series/Report no.: Journal of Biological Chemistry
Abstract: The destination of peroxisomal matrix proteins is encoded by short peptide sequences, which have been characterized as peroxisomal targeting signals (PTS) residing either at the C terminus (PTS1) or close to the N terminus (PTS2). PTS2-carrying proteins interact with their cognate receptor protein PEX7 that mediates their transport to peroxisomes by a concerted action with a co-receptor protein, which in mammals is the PTS1 receptor PEX5L. Using a modified version of the mammalian two-hybrid assay, we demonstrate that the interaction strength between cargo and PEX7 is drastically increased in the presence of the co-receptor PEX5L. In addition, cargo binding is a prerequisite for the interaction between PEX7 and PEX5L and ectopic overexpression of PTS2-carrying cargo protein drastically increases the formation of PEX7-PEX5L complexes in this assay. Consistently, we find that the peroxisomal transfer of PEX7 depends on cargo binding and that ectopic overexpression of cargo protein stimulates this process. Thus, the sequential formation of a highly stable trimeric complex involving cargo protein, PEX7 and PEX5L stabilizes cargo binding and is a prerequisite for PTS2-mediated peroxisomal import.
URI: https://hdl.handle.net/10356/88962
http://hdl.handle.net/10220/46061
ISSN: 0021-9258
DOI: http://dx.doi.org/10.1074/jbc.M114.601575
Rights: © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. This paper was published in Journal of Biological Chemistry and is made available as an electronic reprint (preprint) with permission of The American Society for Biochemistry and Molecular Biology, Inc. The published version is available at: [http://dx.doi.org/10.1074/jbc.M114.601575]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

Files in This Item:
File Description SizeFormat 
Mechanistic insights into PTS2-mediated peroxisomal protein import.pdf2.39 MBAdobe PDFThumbnail
View/Open

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.