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Title: Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids
Authors: Vaschenko, Svetlana
Ramanenka, Andrey
Kulakovich, Olga
Muravitskaya, Alina
Guzatov, Dmitry
Lunevich, Anatoly
Glukhov, Yuri
Gaponenko, Sergey
Keywords: Silver Nanoparticles
DRNTU::Engineering::Electrical and electronic engineering
Pasmon-enhanced Fluorescence
Issue Date: 2016
Source: Vaschenko, S., Ramanenka, A., Kulakovich, O., Muravitskaya, A., Guzatov, D., Lunevich, A., ... Gaponenko, S. (2016). Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids. Procedia Engineering, 140, 57-66. doi : 10.1016/j.proeng.2015.08.1111
Series/Report no.: Procedia Engineering
Abstract: A model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times.
ISSN: 1877-7058
DOI: 10.1016/j.proeng.2015.08.1111
Rights: © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license ( Selection and/or peer-review under responsibility of the scientific committee of Symposium 2015 ICMAT
Fulltext Permission: open
Fulltext Availability: With Fulltext
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