dc.contributor.authorSurya, Wahyu
dc.contributor.authorChooduang, Sivadatch
dc.contributor.authorChoong, Yeu Khai
dc.contributor.authorTorres, Jaume
dc.contributor.authorBoonserm, Panadda
dc.contributor.editorJurat-Fuentes, Juan Luis*
dc.date.accessioned2018-11-02T02:40:31Z
dc.date.available2018-11-02T02:40:31Z
dc.date.issued2016
dc.identifier.citationSurya, W., Chooduang, S., Choong, Y. K., Torres, J., & Boonserm, P. (2016). Binary toxin subunits of Lysinibacillus sphaericus are monomeric and form heterodimers after in vitro activation. PLOS ONE, 11(6), e0158356-. doi:10.1371/journal.pone.0158356en_US
dc.identifier.urihttp://hdl.handle.net/10220/46527
dc.description.abstractThe binary toxin from Lysinibacillus sphaericus has been successfully used for controlling mosquito-transmitted diseases. An activation step shortens both subunits BinA and BinB before their interaction with membranes and internalization in midgut cells, but the precise role of this activation step is unknown. Herein, we show conclusively using three orthogonal biophysical techniques that protoxin subunits form only monomers in aqueous solution. However, in vitro activated toxins readily form heterodimers. This oligomeric state did not change after incubation of these heterodimers with detergent. These results are consistent with the evidence that maximal toxicity in mosquito larvae is achieved when the two subunits, BinA and BinB, are in a 1:1 molar ratio, and directly link proteolytic activation to heterodimerization. Formation of a heterodimer must thus be necessary for subsequent steps, e.g., interaction with membranes, or with a suitable receptor in susceptible mosquito species. Lastly, despite existing similarities between BinB C-terminal domain with domains 3 and 4 of pore-forming aerolysin, no aerolysin-like SDS-resistant heptameric oligomers were observed when the activated Bin subunits were incubated in the presence of detergents or lipidic membranes.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent14 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesPLOS ONEen_US
dc.rights© 2016 Surya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectBinary Toxinen_US
dc.subjectLysinibacillus Sphaericusen_US
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.titleBinary toxin subunits of Lysinibacillus sphaericus are monomeric and form heterodimers after in vitro activationen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0158356
dc.description.versionPublished versionen_US


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