dc.contributor.authorAcerbi, Enzo
dc.contributor.authorViganò, Elena
dc.contributor.authorPoidinger, Michael
dc.contributor.authorMortellaro, Alessandra
dc.contributor.authorZelante, Teresa
dc.contributor.authorStella, Fabio
dc.date.accessioned2018-11-07T07:41:37Z
dc.date.available2018-11-07T07:41:37Z
dc.date.issued2016
dc.identifier.citationAcerbi, E., Viganò, E., Poidinger, M., Mortellaro, A., Zelante, T., & Stella, F. (2016). Continuous time Bayesian networks identify Prdm1 as a negative regulator of TH17 cell differentiation in humans. Scientific Reports, 6, 23128-. doi:10.1038/srep23128en_US
dc.identifier.urihttp://hdl.handle.net/10220/46573
dc.description.abstractT helper 17 (TH17) cells represent a pivotal adaptive cell subset involved in multiple immune disorders in mammalian species. Deciphering the molecular interactions regulating TH17 cell differentiation is particularly critical for novel drug target discovery designed to control maladaptive inflammatory conditions. Using continuous time Bayesian networks over a time-course gene expression dataset, we inferred the global regulatory network controlling TH17 differentiation. From the network, we identified the Prdm1 gene encoding the B lymphocyte-induced maturation protein 1 as a crucial negative regulator of human TH17 cell differentiation. The results have been validated by perturbing Prdm1 expression on freshly isolated CD4+ naïve T cells: reduction of Prdm1 expression leads to augmentation of IL-17 release. These data unravel a possible novel target to control TH17 polarization in inflammatory disorders. Furthermore, this study represents the first in vitro validation of continuous time Bayesian networks as gene network reconstruction method and as hypothesis generation tool for wet-lab biological experiments.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent7 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesScientific Reportsen_US
dc.rights© 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.subjectCell Differentiationen_US
dc.subjectPRDM1 Protein, Humanen_US
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.titleContinuous time Bayesian networks identify Prdm1 as a negative regulator of TH17 cell differentiation in humansen_US
dc.typeJournal Article
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1038/srep23128
dc.description.versionPublished versionen_US


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