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|Title:||New insights into the plasmodium vivax transcriptome using RNA-Seq||Authors:||Zhu, Lei
Day, Nicholas P.
White, Nicholas J.
Preiser, Peter Rainer
|Issue Date:||2016||Source:||Zhu, L., Mok, S., Imwong, M., Jaidee, A., Russell, B., Nosten, F., . . . Bozdech, Z. (2016). New insights into the plasmodium vivax transcriptome using RNA-Seq. Scientific Reports, 6, 20498-. doi:10.1038/srep20498||Series/Report no.:||Scientific Reports||Abstract:||Historically seen as a benign disease, it is now becoming clear that Plasmodium vivax can cause significant morbidity. Effective control strategies targeting P. vivax malaria is hindered by our limited understanding of vivax biology. Here we established the P. vivax transcriptome of the Intraerythrocytic Developmental Cycle (IDC) of two clinical isolates in high resolution by Illumina HiSeq platform. The detailed map of transcriptome generates new insights into regulatory mechanisms of individual genes and reveals their intimate relationship with specific biological functions. A transcriptional hotspot of vir genes observed on chromosome 2 suggests a potential active site modulating immune evasion of the Plasmodium parasite across patients. Compared to other eukaryotes, P. vivax genes tend to have unusually long 5′ untranslated regions and also present multiple transcription start sites. In contrast, alternative splicing is rare in P. vivax but its association with the late schizont stage suggests some of its significance for gene function. The newly identified transcripts, including up to 179 vir like genes and 3018 noncoding RNAs suggest an important role of these gene/transcript classes in strain specific transcriptional regulation.||URI:||https://hdl.handle.net/10356/87477
|DOI:||http://dx.doi.org/10.1038/srep20498||Rights:||© 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
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