Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87490
Title: Resonance raman probes for organelle-specific labeling in live cells
Authors: Kuzmin, Andrey N.
Pliss, Artem
Lim, Chang-Keun
Heo, Jeongyun
Kim, Sehoon
Rzhevskii, Alexander
Gu, Bobo
Yong, Ken-Tye
Wen, Shuangchun
Prasad, Paras N.
Keywords: Raman Spectrometry
Molecular Probes
DRNTU::Engineering::Electrical and electronic engineering
Issue Date: 2016
Source: Kuzmin, A. N., Pliss, A., Lim, C. K., Heo, J., Kim, S., Rzhevskii, A., . . . Prasad, P. N. (2016). Resonance raman probes for organelle-specific labeling in live cells. Scientific Reports, 6, 28483-. doi:10.1038/srep28483
Series/Report no.: Scientific Reports
Abstract: Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging.
URI: https://hdl.handle.net/10356/87490
http://hdl.handle.net/10220/46745
DOI: http://dx.doi.org/10.1038/srep28483
Rights: © 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:EEE Journal Articles

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