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|Title:||Resonance raman probes for organelle-specific labeling in live cells||Authors:||Kuzmin, Andrey N.
Prasad, Paras N.
DRNTU::Engineering::Electrical and electronic engineering
|Issue Date:||2016||Source:||Kuzmin, A. N., Pliss, A., Lim, C. K., Heo, J., Kim, S., Rzhevskii, A., . . . Prasad, P. N. (2016). Resonance raman probes for organelle-specific labeling in live cells. Scientific Reports, 6, 28483-. doi:10.1038/srep28483||Series/Report no.:||Scientific Reports||Abstract:||Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging.||URI:||https://hdl.handle.net/10356/87490
|DOI:||http://dx.doi.org/10.1038/srep28483||Rights:||© 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||EEE Journal Articles|
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