Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87912
Title: Quantitative optical coherence microscopy for the in situ investigation of the biofilm
Authors: Meleppat, Ratheesh Kumar
Shearwood, Christopher
Keey, Seah Leong
Matham, Murukeshan Vadakke
Keywords: Biofilms
Optical Coherence Tomography
DRNTU::Engineering::Mechanical engineering
Issue Date: 2016
Source: Meleppat, R. K., Shearwood, C., Keey, S. L., & Matham, M. V. (2016). Quantitative optical coherence microscopy for the in situ investigation of the biofilm. Journal of Biomedical Optics, 21(12), 127002-. doi:10.1117/1.JBO.21.12.127002
Series/Report no.: Journal of Biomedical Optics
Abstract: This paper explores the potential of optical coherence microscopy (OCM) for the in situ monitoring of biofilm growth. The quantitative imaging of the early developmental biology of a representative biofilm, Klebsiella pneumonia (KP-1), was performed using a swept source-based Fourier domain OCM system. The growth dynamics of the KP-1 biofilms and their transient response under perturbation was investigated using the enface visualization of microcolonies and their spatial localization. Furthermore, the optical density (OD) and planar density of the biofilms are calculated using an OCM technique and compared with OD and colony forming units measured using standard procedures via the sampling of the flow-cell effluent.
URI: https://hdl.handle.net/10356/87912
http://hdl.handle.net/10220/46879
ISSN: 1083-3668
DOI: http://dx.doi.org/10.1117/1.JBO.21.12.127002
Rights: © 2016 Society of Photo-optical Instrumentation Engineers (SPIE). This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-optical Instrumentation Engineers (SPIE). The published version is available at: [http://dx.doi.org/10.1117/1.JBO.21.12.127002]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

Files in This Item:
File Description SizeFormat 
Quantitative optical coherence microscopy for the in situ investigation of the biofilm.pdf2.98 MBAdobe PDFThumbnail
View/Open

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.