Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88607
Title: The Drosophila Dicer-1 partner loquacious enhances miRNA processing from hairpins with unstable structures at the dicing site
Authors: Lim, Mandy Yu Theng
Ng, Alvin Wei Tian
Chou, Yuting
Lim, Teck Por
Simcox, Amanda
Tucker-Kellogg, Greg
Okamura, Katsutomo
Keywords: Drosophila Proteins
RNA Processing
DRNTU::Science::Biological sciences
Issue Date: 2016
Source: Lim, M. Y. T., Ng, A. W. T., Chou, Y., Lim, T. P., Simcox, A., Tucker-Kellogg, G., & Okamura, K. (2016). The Drosophila Dicer-1 partner loquacious enhances miRNA processing from hairpins with unstable structures at the dicing site. Cell Reports, 15(8), 1795-1808. doi:10.1016/j.celrep.2016.04.059
Series/Report no.: Cell Reports
Abstract: In Drosophila, Dicer-1 binds Loquacious-PB (Loqs-PB) as its major co-factor. Previous analyses indicated that loqs mutants only partially impede miRNA processing, but the activity of minor isoforms or maternally deposited Loqs was not eliminated in these studies. We addressed this by generating a cell line from loqs-null embryos and found that only ∼40% of miRNAs showed clear Loqs dependence. Genome-wide comparison of the hairpin structure and Loqs dependence suggested that Loqs substrates are influenced by base-pairing status at the dicing site. Artificial alteration of base-pairing stability at this position in model miRNA hairpins resulted in predicted changes in Loqs dependence, providing evidence for this hypothesis. Finally, we found that evolutionarily young miRNA genes tended to be Loqs dependent. We propose that Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates. Graphical Abstract
URI: https://hdl.handle.net/10356/88607
http://hdl.handle.net/10220/46946
ISSN: 2211-1247
DOI: http://dx.doi.org/10.1016/j.celrep.2016.04.059
Rights: © 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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