Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88999
Title: The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis
Authors: Ivanyi-Nagy, Roland
Ahmed, Syed Moiz
Peter, Sabrina
Ramani, Priya Dharshana
Ong, Peh Fern
Dreesen, Oliver
Dröge, Peter
Keywords: DRNTU::Science::Biological sciences
Homeostasis
Telomerase RNA
Issue Date: 2018
Source: Ivanyi-Nagy, R., Ahmed, S. M., Peter, S., Ramani, P. D., Ong, P. F., Dreesen, O., & Dröge, P. (2018). The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis. eLife, 7, e40037-. doi: 10.7554/eLife.40037
Series/Report no.: eLife
Abstract: Telomerase RNA (TR) provides the template for DNA repeat synthesis at telomeres and is essential for genome stability in continuously dividing cells. We mapped the RNA interactome of human TR (hTR) and identified a set of non-coding and coding hTR-interacting RNAs, including the histone 1C mRNA (HIST1H1C). Disruption of the hTR-HIST1H1C RNA association resulted in markedly increased telomere elongation without affecting telomerase enzymatic activity. Conversely, over-expression of HIST1H1C led to telomere attrition. By using a combination of mutations to disentangle the effects of histone 1 RNA synthesis, protein expression, and hTR interaction, we show that HIST1H1C RNA negatively regulates telomere length independently of its protein coding potential. Taken together, our data provide important insights into a surprisingly complex hTR-RNA interaction network and define an unexpected non-coding RNA role for HIST1H1C in regulating telomere length homeostasis, thus offering a glimpse into the mostly uncharted, vast space of non-canonical messenger RNA functions.
URI: https://hdl.handle.net/10356/88999
http://hdl.handle.net/10220/47009
DOI: http://dx.doi.org/10.7554/eLife.40037
Rights: © 2018 Ivanyi-Nagy et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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