Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/90215
Title: Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices
Authors: Ramalingam, Naveen
Warkiani, Majid Ebrahimi
Gong, Thomas Hai-Qing
Keywords: DRNTU::Engineering::Mechanical engineering
Polymers
Amplicons
Issue Date: 2017
Source: Ramalingam, N., Warkiani, M. E., & Gong, T. H.-Q. (2017). Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices. Biomicrofluidics, 11(3), 034110-. doi:10.1063/1.4983692
Series/Report no.: Biomicrofluidics
Abstract: Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices. Our study results reveal that the use of acetylated BSA in PCR micro-devices leads to differential inhibition of PCR, compared to non-acetylated BSA. This result is noticed for the first time, and the differential inhibition generally goes un-noticed, as compared to complete PCR inhibition.
URI: https://hdl.handle.net/10356/90215
http://hdl.handle.net/10220/47244
DOI: 10.1063/1.4983692
Rights: © 2017 The Author(s) (Published by AIP). This paper was published in Biomicrofluidics and is made available as an electronic reprint (preprint) with permission of The Author(s) (Published by AIP). The published version is available at: [http://dx.doi.org/10.1063/1.4983692]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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