dc.contributor.authorLim, Yan Ting
dc.contributor.authorPrabhu, Nayana
dc.contributor.authorDai, Lingyun
dc.contributor.authorGo, Ka Diam
dc.contributor.authorChen, Dan
dc.contributor.authorSreekumar, Lekshmy
dc.contributor.authorEgeblad, Louise
dc.contributor.authorEriksson, Staffan
dc.contributor.authorChen, Liyan
dc.contributor.authorVeerappan, Saranya
dc.contributor.authorTeo, Hsiang Ling
dc.contributor.authorTan, Chris Soon Heng
dc.contributor.authorLengqvist, Johan
dc.contributor.authorLarsson, Andreas
dc.contributor.authorSobota, Radoslaw M.
dc.contributor.authorNordlund, Pär
dc.contributor.editorLau, Andy T. Y.*
dc.identifier.citationLim, Y. T., Prabhu, N., Dai, L., Go, K. D., Chen, D., Sreekumar, L., . . . Nordlund, P. (2018). An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions. PLOS ONE, 13(12), e0208273-. doi:10.1371/journal.pone.0208273en_US
dc.description.abstractMetabolite-protein interactions define the output of metabolic pathways and regulate many cellular processes. Although diseases are often characterized by distortions in metabolic processes, efficient means to discover and study such interactions directly in cells have been lacking. A stringent implementation of proteome-wide Cellular Thermal Shift Assay (CETSA) was developed and applied to key cellular nucleotides, where previously experimentally confirmed protein-nucleotide interactions were well recaptured. Many predicted, but never experimentally confirmed, as well as novel protein-nucleotide interactions were discovered. Interactions included a range of different protein families where nucleotides serve as substrates, products, co-factors or regulators. In cells exposed to thymidine, a limiting precursor for DNA synthesis, both dose- and time-dependence of the intracellular binding events for sequentially generated thymidine metabolites were revealed. Interactions included known cancer targets in deoxyribonucleotide metabolism as well as novel interacting proteins. This stringent CETSA based strategy will be applicable for a wide range of metabolites and will therefore greatly facilitate the discovery and studies of interactions and specificities of the many metabolites in human cells that remain uncharacterized.en_US
dc.format.extent30 p.en_US
dc.relation.ispartofseriesPLOS ONEen_US
dc.rights© 2018 Lim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectProtein-nucleotide Interactionsen_US
dc.subjectDRNTU::Science::Biological sciencesen_US
dc.titleAn efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactionsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US

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