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|Title:||Comparative genomics of clinical strains of Pseudomonas aeruginosa strains isolated from different geographic sites||Authors:||Subedi, Dinesh
Vijay, Ajay Kumar
Kohli, Gurjeet Singh
Rice, Scott A.
Rice, Scott A
|Issue Date:||2018||Source:||Subedi, D., Vijay, A. K., Kohli, G. S., Rice, S. A., & Willcox, M. (2018). Comparative genomics of clinical strains of Pseudomonas aeruginosa strains isolated from different geographic sites. Scientific Reports, 8(1), 15668-. doi:10.1038/s41598-018-34020-7||Series/Report no.:||Scientific Reports||Abstract:||The large and complex genome of Pseudomonas aeruginosa, which consists of significant portions (up to 20%) of transferable genetic elements contributes to the rapid development of antibiotic resistance. The whole genome sequences of 22 strains isolated from eye and cystic fibrosis patients in Australia and India between 1992 and 2007 were used to compare genomic divergence and phylogenetic relationships as well as genes for antibiotic resistance and virulence factors. Analysis of the pangenome indicated a large variation in the size of accessory genome amongst 22 stains and the size of the accessory genome correlated with number of genomic islands, insertion sequences and prophages. The strains were diverse in terms of sequence type and dissimilar to that of global epidemic P. aeruginosa clones. Of the eye isolates, 62% clustered together within a single lineage. Indian eye isolates possessed genes associated with resistance to aminoglycoside, beta-lactams, sulphonamide, quaternary ammonium compounds, tetracycline, trimethoprims and chloramphenicols. These genes were, however, absent in Australian isolates regardless of source. Overall, our results provide valuable information for understanding the genomic diversity of P. aeruginosa isolated from two different infection types and countries.||URI:||https://hdl.handle.net/10356/103319
|DOI:||http://dx.doi.org/10.1038/s41598-018-34020-7||Rights:||© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.||metadata.item.grantfulltext:||open||metadata.item.fulltext:||With Fulltext|
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