dc.contributor.authorZhang, Hui
dc.contributor.authorYu, Ting
dc.contributor.authorWang, Yiran
dc.contributor.authorLi, Jie
dc.contributor.authorWang, Guangli
dc.contributor.authorMa, Yingqun
dc.contributor.authorLiu, Yu
dc.identifier.citationZhang, H., Yu, T., Wang, Y., Li, J., Wang, G., Ma, Y., & Liu, Y. (2018). 4-Chlorophenol oxidation depends on the activation of an AraC-type transcriptional regulator, CphR, in Rhodococcus sp. strain YH-5B. Frontiers in Microbiology, 9, 2481-. doi:10.3389/fmicb.2018.02481en_US
dc.description.abstract4-Chlorophenol (4-CP) oxidation plays an essential role in the detoxification of 4-CP. However, oxidative regulation of 4-CP at the genetic and biochemical levels has not yet been studied. To explore the regulation mechanism of 4-CP oxidation, a novel gene cluster, cphRA2A1, involved in biodegradation of 4-CP was identified and cloned from Rhodococcus sp. strain YH-5B by genome walking. The sequence analysis showed that the cphRA2A1 gene cluster encoded an AraC-type transcriptional regulator and a two-component monooxygenase enzyme, while quantitative real-time PCR analysis further revealed that cphR was constitutively expressed and positively regulated the transcription of cphA2A1 genes in response to 4-CP or phenol, as evidenced by gene knockout and complementation experiments. Through the transcriptional fusion of the mutated cphA2A1 promoter with the lacZ gene, it was found that the CphR regulator binding sites had two 15-bp imperfect direct repeats (TGCA-N6-GGNTA) at −35 to −69 upstream of the cphA2A1 transcriptional start site. Notably, the sub-motifs at the −46 to −49 positions played a critical role in the appropriate interaction with the CphR dimer. In addition, it was confirmed that the monooxygenase subunits CphA1 and CphA2, which were purified by His-tag affinity chromatography, were able to catalyze the conversion of 4-CP to 4-chlorocatechol, suggesting that strain YH-5B could degrade 4-CP via the 4-chlorocatechol pathway. This study enhances our understanding of the genetic and biochemical diversity in the transcriptional regulation of 4-CP oxidation in Gram-positive bacteria.en_US
dc.format.extent13 p.en_US
dc.relation.ispartofseriesFrontiers in Microbiologyen_US
dc.rights© 2018 Zhang, Yu, Wang, Li, Wang, Ma and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subject4-Chlorophenol Degradationen_US
dc.subjectGene Clusteren_US
dc.subjectDRNTU::Engineering::Environmental engineeringen_US
dc.title4-Chlorophenol oxidation depends on the activation of an AraC-type transcriptional regulator, CphR, in Rhodococcus sp. strain YH-5Ben_US
dc.typeJournal Article
dc.contributor.researchNanyang Environment and Water Research Instituteen_US
dc.contributor.researchAdvanced Environmental Biotechnology Centre (AEBC)
dc.contributor.schoolSchool of Civil and Environmental Engineeringen_US
dc.description.versionPublished versionen_US

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